ReDisulphID

a tool for identifying drug-targetable redox-active disulphides

Green fluorescent protein

Intermolecular
Cysteine 75 and cysteine 122 of T-cell surface glycoprotein CD3 epsilon chain
Cysteine 72 and cysteine 119 of T-cell surface glycoprotein CD3 epsilon chain
Cysteine 83 and cysteine 96 of Glutamate receptor ionotropic, kainate 2
Cysteine 83 and cysteine 347 of Glutamate receptor ionotropic, kainate 2
A redox-regulated disulphide may form between cysteine 75 of Green fluorescent protein and cysteine 122 of T-cell surface glycoprotein CD3 epsilon chain (75 and 100 respectively in this structure).

Details

Redox score ?
65
PDB code
7phr
Structure name
structure of a fully assembled t-cell receptor engaging a tumor- associated peptide-mhc i
Structure deposition date
2021-08-18
Thiol separation (Å)
5
Half-sphere exposure sum ?
70
Minimum pKa ?
nan
% buried
nan
Peptide A name
Green fluorescent protein
Peptide B name
T-cell surface glycoprotein CD3 epsilon chain
Peptide A accession
P42212
Peptide B accession
P07766
Peptide A residue number
75
Peptide B residue number
122

Ligandability

Cysteine 75 of Green fluorescent protein

Cysteine 122 of T-cell surface glycoprotein CD3 epsilon chain

Cysteine 75 in protein A could not be asigned to a Uniprot residue.
A redox-regulated disulphide may form between cysteine 72 of Green fluorescent protein and cysteine 119 of T-cell surface glycoprotein CD3 epsilon chain (72 and 97 respectively in this structure).

Details

Redox score ?
62
PDB code
7phr
Structure name
structure of a fully assembled t-cell receptor engaging a tumor- associated peptide-mhc i
Structure deposition date
2021-08-18
Thiol separation (Å)
6
Half-sphere exposure sum ?
75
Minimum pKa ?
nan
% buried
nan
Peptide A name
Green fluorescent protein
Peptide B name
T-cell surface glycoprotein CD3 epsilon chain
Peptide A accession
P42212
Peptide B accession
P07766
Peptide A residue number
72
Peptide B residue number
119

Ligandability

Cysteine 72 of Green fluorescent protein

Cysteine 119 of T-cell surface glycoprotein CD3 epsilon chain

Cysteine 72 in protein A could not be asigned to a Uniprot residue.
A redox-regulated disulphide may form between cysteine 83 of Green fluorescent protein and cysteine 96 of Glutamate receptor ionotropic, kainate 2. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
42
PDB code
7ks0
Structure name
gluk2/k5 with 6-cyano-7-nitroquinoxaline-2,3-dione (cnqx)
Structure deposition date
2020-11-20
Thiol separation (Å)
9
Half-sphere exposure sum ?
69
Minimum pKa ?
nan
% buried
nan
Peptide A name
Green fluorescent protein
Peptide B name
Glutamate receptor ionotropic, kainate 2
Peptide A accession
P42212
Peptide B accession
P42260
Peptide A residue number
83
Peptide B residue number
96

Ligandability

Cysteine 83 of Green fluorescent protein

Cysteine 96 of Glutamate receptor ionotropic, kainate 2

Cysteine 83 in protein A could not be asigned to a Uniprot residue.
A redox-regulated disulphide may form between cysteine 83 of Green fluorescent protein and cysteine 347 of Glutamate receptor ionotropic, kainate 2. However, the redox score of this cysteine pair is lower than any known redox-active intermolecular disulphide. ?

Details

Redox score ?
42
PDB code
7ks0
Structure name
gluk2/k5 with 6-cyano-7-nitroquinoxaline-2,3-dione (cnqx)
Structure deposition date
2020-11-20
Thiol separation (Å)
9
Half-sphere exposure sum ?
67
Minimum pKa ?
nan
% buried
nan
Peptide A name
Green fluorescent protein
Peptide B name
Glutamate receptor ionotropic, kainate 2
Peptide A accession
P42212
Peptide B accession
P42260
Peptide A residue number
83
Peptide B residue number
347

Ligandability

Cysteine 83 of Green fluorescent protein

Cysteine 347 of Glutamate receptor ionotropic, kainate 2

Cysteine 83 in protein A could not be asigned to a Uniprot residue.
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